Cho Endonuclease Functions during DNA Interstrand Cross-Link Repair in Escherichia coli.

Genes Affecting the Repair and Survival of Escherichia coli Following Psoralen-Induced Damage: a DNA Interstrand Crosslinking Agent by Anthonige

Recruitment and positioning determine the specific role of the XPF‐ERCC1 endonuclease in interstrand crosslink repair

XPF-ERCC1 is a structure-specific endonuclease pivotal for several DNA repair pathways and, when mutated, can cause multiple diseases. Although the disease-specific mutations are thought to affect different DNA repair pathways, the molecular basis for this is unknown. Here we examine the function of XPF-ERCC1 in DNA interstrand crosslink (ICL) repair. We used Xenopus egg extracts to measure bot...

Identification of KIAA1018/FAN1, a DNA Repair Nuclease Recruited to DNA Damage by Monoubiquitinated FANCD2

DNA interstrand crosslinks (ICLs) are highly toxic because they block the progression of replisomes. The Fanconi Anemia (FA) proteins, encoded by genes that are mutated in FA, are important for repair of ICLs. The FA core complex catalyzes the monoubiquitination of FANCD2, and this event is essential for several steps of ICL repair. However, how monoubiquitination of FANCD2 promotes ICL repair ...

RPA activates the XPF‐ERCC1 endonuclease to initiate processing of DNA interstrand crosslinks

During replication-coupled DNA interstrand crosslink (ICL) repair, the XPF-ERCC1 endonuclease is required for the incisions that release, or "unhook", ICLs, but the mechanism of ICL unhooking remains largely unknown. Incisions are triggered when the nascent leading strand of a replication fork strikes the ICL Here, we report that while purified XPF-ERCC1 incises simple ICL-containing model repl...

Repair of interstrand cross-links in DNA induced by psoralen plus light.

The clinical drug psoralen, in the presence of 360-nm light, binds to cellular DNA, forming monofunctional adducts with pyrimidine bases and interstrand cross-links. Cross-links, however, rather than monoadducts, appear to be the DNA lesion primarily responsible for the sensitizing action of this drug. Formation of an interstrand cross-link necessarily damages both DNA strands at nearly the sam...